Description
The REALQUALITY RQ-ACE (I/D) kit is an IVD for detection and genotyping of the insertion/deletion (I/D) polymorphism of a 289 bp Alu repeat sequence in intron 16 of the human coding gene for the Angiotensin-Converting Enzyme (ACE) by Real-Time PCR.
Product Characteristics
- The assay requires only 1 µL of DNA extracted
- Compatible with the most common Real-Time PCR instruments
- It is equipped with a dUTP/UNG system for the prevention of carry-over contamination and a fluorescence normalizer
- The automatic format of the assay can be used on GENEQUALITY® automatic platforms
- It includes an automatic interpretation of results with AB SNP Report software
Kit content
Kit content:
- Ready-to-use reagents for Real-Time PCR
- Homozygous (I/I and D/D) and heterozygous (I/D) positive controls
Further Information
The angiotensin-converting enzyme (ACE) is a zinc metallopeptidase that is abundantly found on the surface of epithelial and endothelial cells. ACE plays a crucial role in two separate physiological systems. It converts angiotensin I to angiotensin II, a strong vasoconstrictor, and degrades bradykinin, a potent vasodilator. pressure by regulating the volume of fluids in the body.
The plasma levels of ACE vary strongly between different individuals and several studies suggest that these variations can be attributed to approx. 50% to an insertion/deletion (I/D) polymorphism in intron 16 of the ACE gene.
This polymorphism is defined by the presence (insertion, allele I) or absence (deletion, allele D) of a 289 bp Alu-repeat sequence. The following three distinct genotypes can be found: I/I = homozygous for the insertion; I/D = heterozygous for the insertion and deletion; D/D = homozygous for the deletion.
Various studies have demonstrated the D allele to be associated with higher plasma and tissue levels of ACE, which are doubled in subjects carrying the D allele compared to subjects homozygous for the I allele (I/I). The carriers of the D allele also have an increased risk of endothelial damage and developing hypercoagulation and cardiovascular disorders. A connection between presence of the D allele and atherosclerosis, mainly due to an altered carotid artery intima-media thickness (CIMT), has been confirmed by further studies.
Ordering Information
| Code | Product | PKG |
|---|---|---|
| RQ-75-4M | REALQUALITY RQ-ACE (I/D) | 50 tests |
| RQ-75-6M | REALQUALITY RQ-ACE (I/D) | 100 tests |
| RQ-75-4A | REALQUALITY RQ-ACE (I/D) - Automatic format | 50 tests |
| RQ-75-6A | REALQUALITY RQ-ACE (I/D) - Automatic format | 100 tests |