REALQUALITY RQ-MBT Complex

Description

The REALQUALITY RQ-MBT Complex is an IVD device for detection and quantification of the DNA of Mycobacterium tuberculosis complex (MBTC) by Real-Time PCR

Product Characteristics

• The device is validated on DNA extracted from various samples types
• The assay requires only 5 µL of DNA extracted
• If used in combination with the REALQUALITY RQ-MBT Complex STANDARD, it allows the quantification of the bacterial DNA present in the sample
• The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits
• Validated on main Real-Time PCR instruments
• The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
• Easy interpretation of results with AB Genius Report software

Kit content

Kit contains:

• Ready-to-use reagents for Real-Time PCR
• Internal control
• Positive control

Further Information

Tuberculosis, in its most widespread and known form, is pulmonary tuberculosis (which makes up about 73% of cases), although in reality almost all organs can be involved Mycobacterium tuberculosis is an intracellular pathogen that involves peripheral blood macrophages and monocytes, peripheral tissue macrophages, promonocytes, and bone marrow monocytes during infection.
In immunocompetent subjects the mycobacterium-macrophage interaction leads to an activation of the macrophage itself with consequent bacterial lysis due to enzymatic digestion and oxidation processes. In immunocompromised subjects, on the contrary, the inability to perform a bactericidal action is considered the main cause of the systemic spread of the mycobacterium. The histopathological lesions observed can be of the exudative type, characterized by acute inflammation, formation of exudate and accumulation of PMN leukocytes around the bacilli, and productive (granulomatous) lesions in which macrophages are distributed concentrically (epithelioid cells) to form tubercles and cells giants.

Since amplification tests are not screening tests, their use must be limited to cases with suspicion of active tuberculous disease supported by clinical and/or radiological evidence. By their very nature, nucleic acid amplification tests are not linked to the viability of the microorganism and can remain positive even after culture negativization and even after completion of therapy; they must therefore be used exclusively in the diagnostic phase and not in the therapeutic follow-up.

Ordering Information